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If you were using Keyfile along with the Master Password, then both they are required to decrypt your data. Actually, the text from the Keyfile gets appended to the master password (to make it extra strong and unguessable) and together they create a new Master Password to start the encryption. So the impact of losing keyfile is same as forgetting Master Password, and you need to start over by resetting your Enpass this way which will result in the loss of all your existing data.